De Rossi was dismissed and Ivan Juric was appointed as the new coach at Roma on Wednesday, with Souloukou playing a significant role in the decision-making process.
On Friday, at the Trigora training ground, ultras hung a banner making clear their feelings on the decision.
It read: ‘De Rossi, the sea of Rome. Lina the evil of Rome.’
Roma and Juric will meet Udinese on Sunday.